DDK egg-foreign sperm incompatibility in mice is not between the pronuclei.
نویسنده
چکیده
A high rate of normal postimplantation development was achieved when the pronuclei of embryos from matings of DDK females with (CBA X C57BL/6J)F1 males were transplanted into enucleated embryos of non-DDK origin. This shows that the DDK egg cytoplasm, not the maternal pronucleus, is involved in the late preimplantation-lethal incompatibility.
منابع مشابه
Reduced gap junctional communication is associated with the lethal condition characteristic of DDK mouse eggs fertilized by foreign sperm.
Communication through gap junctions was examined in 8-cell zygotes generated by fertilization of eggs of the DDK inbred strain of mice with spermatozoa of the C3H strain. These zygotes spontaneously begin to extrude cells at the late 16-cell stage and 95% die by the blastocyst stage. The transfer of Lucifer Yellow between cells of DDK/C3H zygotes that had not yet begun to express the defect was...
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The fertility of DDK mice was investigated in reciprocal crosses between DDK and C57BL/6J strains of mice. When mated to C57BL males, DDK females were semi-sterile. By contrast, when C57BL females were mated to DDK males, they showed better fertility than in the intra-strain mating. Embryological study has revealed that the low fertility of DDK females is caused by embryonic death at the morula...
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Insemination of sea urchin (Arbacia) ova with mussel (Mytilus) sperm has been accomplished by treating eggs with trypsin and suspending the gametes in seawater made alkaline with NaOH. Not all inseminated eggs undergo a cortical granule reaction. Some eggs either elevate what remains of their vitelline layer or demonstrate no cortical modification whatsoever. After its incorporation into the eg...
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The main question addressed in this thesis is what happens between the moment when the sperm enters the egg and the fusion of the male and the female pronuclei. Orientation of the apposing pronuclei most likely plays a decisive role in the polarity of the developing embryo. The migration and the dependence between the pronuclei have been investigated through three different measures of correlat...
متن کاملThe paternal gene of the DDK syndrome maps to the Schlafen gene cluster on mouse chromosome 11.
The DDK syndrome is an early embryonic lethal phenotype observed in crosses between females of the DDK inbred mouse strain and many non-DDK males. Lethality results from an incompatibility between a maternal DDK factor and a non-DDK paternal gene, both of which have been mapped to the Ovum mutant (Om) locus on mouse chromosome 11. Here we define a 465-kb candidate interval for the paternal gene...
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ورودعنوان ژورنال:
- Journal of reproduction and fertility
دوره 76 2 شماره
صفحات -
تاریخ انتشار 1986